Single-gene target detection systems could, in this case, lead to a false negative signal. Yet, it has now been documented that Salmonella can lose an entire Pathogenicity island while remaining infectious (Hu et al. invA for Salmonella or hlyA for Listeria detection). Moreover, current detection assays are mostly based on the detection of a single virulence gene (e.g. TaqMan® and SYBR®Green), they are using different protocols and they were developed for different purposes and validated following different ways. However, their use all together into the same detection system is hampered by several drawbacks: they are using different technologies (e.g. 2011), and several qPCR assays have already been developed (e.g. Real-time PCR (qPCR) assays have gained confidence as faster and reliable alternatives (Postollec et al. Therefore, developing fast and simple foodborne pathogens detection tools, able to simultaneously detect several pathogens, remains a not only necessity but also a real challenge. at least 5 days for Salmonella detection (ISO 6579 norm 2002)). They are reliable but focus on individual pathogenic species, are time consuming and labour intensive (e.g. Currently, the official methods for the detection of pathogenic bacteria in food samples are norms from the International Organization for Standardization (ISO). The recent outbreak of the O104:H4 serotype of Escherichia coli in Germany was a dramatic illustration of all the consequences of a severe outbreak (European Commission 2011). Foodborne diseases cause not only an enormous economic burden due to sick leaves, treatments, hospitalisations and mortality (Scharff 2012) but also economical losses of farmers and industry. The food safety is an important concern worldwide.
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